Analysis,identification and strategies on interferences in immunoassay

VernacularTitle:免疫检测干扰因素的分析、识别和对策
Author: Gusheng TANG ; Yu WU ; Qian SHEN
Publication Type:Journal Article
Keywords: Games,experimental; Immunoassays; Test interference; Interferenceidentification
From: Chinese Journal of Laboratory Medicine 2009;32(7):725-729
CountryChina
Language:Chinese
Abstract: Substances that might potentially alter the measurable concentration of the analyte or alter the binding ability of detection antibody can lead to immunoassay interference. Endogenous interferences consist of autoantibodies, heterophiles antibodies, human anti-animal antibodies (HAAA) and some binding proteins. Lipidemia, cross-reactivity, pre-analytical variation, matrix and different detection equipments may also affect immunoassay. These interfering substances may cause falsely increased or decreased concentration in many analytes, including hormones, tumor markers, drugs, cardiac tropanin and microbial serology, thus it will affect the diagnosis of patients and the evaluation of therapeutic strategies Laboratorians and physicians should both be aware of the potential interference in immunoassays and communicate closely when any clinical discordance between the clinical and the laboratory data appears, avoiding a subsequent wrong diagnosis and unwarranted treatment. In this case, an alternative assay or measurement is needed for the potential correct results.