Effect of oxymatrine on the expression of p-STAT3 and PIAS3 in human mesangial cells
10.3760/cma.j.issn.1001-7097.2009.08.010
- VernacularTitle:苦参素对人肾小球系膜细胞磷酸化信号传导和转录激活因子3及其蛋白抑制剂表达的影响
- Author:
Hongxing DANG
;
Yu JIN
;
Yuning LI
;
Jizu LING
;
Jie SU
- Publication Type:Journal Article
- Keywords:
Oxymatrine;
Mesangial cells;
Cell proliferation;
STAT3 transcription factor;
Protein inhibitors of activated STAT;
Signal transduction
- From:
Chinese Journal of Nephrology
2009;25(8):635-639
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of oxymatrine on p-STAT3 and PIAS3 signaling molecule and it's mRNA expression in the proliferation of the human mesangial cells (HMCs) induced by lipopolysaccharide(LPS) and to explore their relationship. Methods HMCs were divided into three groups: control group, LPS group and oxymatrine group. HMC proliferation was detected by methyl thiazolyl tetrazolium (MTT) assay. Type Ⅳ collagen in the supernatants of the cultured HMCs was detected by ELISA at 12, 24, 48 hours respectively. At the same time, the protein and mRNA expressions of p-STAT3 and PIAS3 were measured by Western blot and real-time quantitative RT-PCR. Results The cell proliferation, the mRNA and protein expression of type Ⅳ collagen, p-STAT3 in LPS group were increased compared with the control group (P<0.01), but they were decreased in oxymatrine group (P < 0.01). The expressions of protein and mRNA of PIAS3 in LPS group were decreased significantly compared with control group (P<0.01), but they were increased in oxymatrine group (P<0.01). Conclusion Oxymatrine can down-regulate the expression of p-STAT3 and up-regulate the expression of PIAS3, which plays an important role in the process of LPS-induced HMCs proliferation.
