The effects of rehabilitation training on learning, memory and expression of GAP-43 in hippocampus CA1 area of rats with vascular dementia
10.3760/cma.j.issn.0254-1424.2009.07.001
- VernacularTitle:运动训练对血管性痴呆大鼠学习记忆能力及海马GAP-43表达的影响
- Author:
Zhenyong FAN
;
Lina CHENG
;
Linfeng XU
;
Ya ZONG
;
Jianyong HU
;
Xianghua YU
;
Weizhong GU
- Publication Type:Journal Article
- Keywords:
Vascular dementia;
Rehabilitation training;
Learning and memory;
Growth-associated protein
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2009;31(7):433-436
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of rehabilitation training on learning and memory ability and the expression of growth-associated protein-43(GAP-43)in rats with vascular dementia.Methods Forty-four female Sprague-Dawley rats were randomly assigned to a rehabilitation group(n=20),an immobilization group(n=20),and a sham-operation group(n=4).The rats in the former 2 groups were operated on to establish the experimental vascular dementia model by repeatedly ischemia/reperfusion injury of brain induced by ligation of bilateral common carotid arteries and lowering of blood pressure induced by intraabdominal injection of sodium nitroprusside.The rats in rehabilitation group were administered with rotating bar and rolling cage exercises for 1 hour once daily,while those in the immobilization group were immobilized and without any exercise:the rats in sham-operation group could move freely in cage.Learning and memory tests were preformed by using step-down avoidance test at the 27th and 28th days after operation.Immunohistochemical staining was used to detect GAP-43 expression in hippocampus CA1 area at different time points after operation. Results The rats in rehabilitation group demonstrated better learning and memory ability than those in immobilization group(P<0.01),and more GAP-43 expression in hippocampus CA1 ar-ea than those in immobilization group and sham-operation(P<0.01).Conclusion Rehabilitation training can im-prove the learning and memory ability of rats with experimental vascular dementia,and the mechanism is probably re-lated to the increase of GAP-43 in hippocampus CA1 area.
