Effects of RNAi on keloid formation by inhibiting heat shock protein
10.3760/cma.j.issn.1671-0290.2009.05.011
- VernacularTitle:RNA干扰技术阻抑热休克蛋白对瘢痕疙瘩生成的影响
- Author:
Peisheng JIN
;
Junjie CHEN
;
Ying CEN
;
Aijun ZHANG
;
Changbo TAO
;
Xueyang LI
- Publication Type:Journal Article
- Keywords:
RNA interference;
Heat shock protein 47;
Keloid;
Interference in vivo
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2009;15(5):327-330
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the significance of HSP47 gene in keloid formation after in vivo study with RNAi technology and the recombinant HSP4 7 siRNA against heat shock protein 47 to keloid in a nude mice model.Methods We injected RNAi mixture into the keloid of a nude mice model in experimental group and PBS water(0.25 ml)into control group at the 16th days after establishing the models.After interference we observed the specimens and harvested specimens at 7th days for biochemical and pathological analysis.Results The expression of HSP47 mRNA reduced obviously and the collagen content also reduced in the experimental group.The rusults had statistical significance.Conclusion We can suppress the expression of HSP47 gene and then reduce the production of collagen after in vivo interfering experiment with HSP4 7 siRNA in keloid nude mice models using RNAi technique.This study cornfirms the mechanism that HSP47 promotes the keloid formation,which provides a new target to treat keloid.
