Interleukin-1β promotes the expression of pSTAT3 in rat retinal Müller cells
10.3760/cma.j.issn.1005-1015.2009.05.16
- VernacularTitle:白细胞介素-1β对大鼠视网膜Müller细胞磷酸化的信号转导及转录活化因子3表达的影响
- Author:
Xi SHEN
;
Gezhi XU
- Publication Type:Journal Article
- Keywords:
Interleukin-1beta/administration & dosage;
Janus kinases/pathophysiology;
MOiler cell
- From:
Chinese Journal of Ocular Fundus Diseases
2009;25(5):385-388
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influence of interleukin-1β (IL-1β) on the expression of phosphorylated signal transducers and activators of transcription 3 (pSTAT 3) in rat retinal MOiler cells. Methods For in vitro study cultured Mailer cells were treated with IL-1β of different concentrations (0, 0.1, 1, 5 and 10 ng/ml) for 24 hours. For in vivo study, 32 Sprague-Dawley(SD)rats were divided into 4 groups randomly (control group, 100,500 and 1000 ng/ml group) with 8 rats in each group. After 24 hours of injection with phosphate buffered solution (PBS), or 100, 500, 1000 ng/ml IL-1β into the vitreous treated retinas were evaluated by indirect immunofluorescence and western blotting. Results After 24 hours of incubation without IL-1β, pSTAT3 has little expression in cultured Muller cells, but was up-regulated by 1 ng/ml or higher IL-1β in a dosage-dependent manner (F=46.64, 43.78; P<0.01). pSTAT3 was not expressed in adult rat retina, but was up-regulated by vitreous injection of 100 ng/ml or higher IL-1β in a dosage-dependent manner (F=73.53, 43.70; P<0.01). pSTAT3 expressed mainly in inner nuclear layer and ganglion cell layer. Double-labeling showed that there was no co-staining of pSTAT3 and glial fibrillary treated with IL-1β. Conclusions Expression of pSTAT3 in MUller cells could be activated by IL-1β which may represent one pathway link to reactive gliosis.
