Analyses of the TCR β-chain gene rearrangement and CDR3 repertoire in patients with peripheral T-cell lymphoma
10.3760/cma.j.issn.1006-9801.2009.10.003
- VernacularTitle:外周T细胞淋巴瘤TCR基因重排及CDR3谱型分析
- Author:
Jianbo ZHANG
;
Yongping SONG
;
Qingkai YU
;
Ruihua BAI
;
Wei SONG
;
Jun HU
;
Tao DONG
- Publication Type:Journal Article
- Keywords:
Gene rearrangement;
Complementarity determining regions;
Lymphoma;
T-cell;
Genescan
- From:
Cancer Research and Clinic
2009;21(10):657-659
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the elonal gene rearrangement and complementarity determining region 3 (CDR3) Repertoire of TCR β-chain in fine needle aspiration biopsy (FNAB) specimens of peripheral T-cell lymphoma. Methods The TCR CDR3 region genes of 24 TCR Vβ subfamilies were amplified by utilizing RT-PCR technology, and the CDR3 size lengths of TCR β-chain were analyzed with genesean technology for 4 healthy individuals and 4 patients with peripheral T-cell lymphoma. The clonality of T cells presumed by spectratyping was further confirmed by CDR3 sequencing. Results TCR β-chain presented specific repertoire skewing in 4 cases with peripheral T-cell lymphoma, and only 1-4 TCR Vβ subfamily T cells were identified, respectively. Clonal expanded T cells, including mono, bioclonal and oligoclonal trend patterns, in one or more Vβ subfamilies were found in all cases. The mono expanded T cells have different CDR3 amino acid sequences. Conclusion Characteristic T cells cloning proliferation and selected usage of TCR Vβ subfamily T cells were found in 4 cases with peripheral T-cell lymphoma. The sequences of CDR3 in different TCR clone proliferation are different.
