Effects of insulin-like growth factor-1 on expression of stem cell factor in gastric smooth muscle cells
10.3760/cma.j.issn.0254-1432.2009.10.002
- VernacularTitle:胰岛素样生长因子-1对胃平滑肌细胞合成干细胞因子的作用
- Author:
Hui LI
;
Lin LIN
;
Yueji NING
;
Wei ZHANG
;
Liming XU
;
Xueliang LI
- Publication Type:Journal Article
- Keywords:
Insulin-like growth factor-1;
Myocytes,smooth muscle;
Stem cell factor Antibodies
- From:
Chinese Journal of Digestion
2009;29(10):653-657
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of exogenous insulin-like growth factor-1(IGF-1)on growth of gastric smooth muscle cells(SMCs)and synthesis of stem cell factor(SCF).Methods Gastric SMCs were cultured by enzymolysis and were identified by α-actin immunocytochemical method.After that,SMCs were incubated with different concentrations of IGF-1(0,50,100,150μg/L)or IGF-1 receptor monoclonal antibody(0.50,100,150μg/L)for 24 hours.Proliferation of SMCs was measured by MTT.The expressions of SCF protein and RNA were analyzed by Western blotting and quantitative reverse transcription-polymerase chain reaction,respectively.The SCF content in the culture fluid was detected by enzyme-linked immunosorbent assay(ELISA).Results The proliferation of gastric SMCs and the expression of SCF can be enhanced by IGF-1,and 100μg/L of IGF-1 may be the final effective concentration.Nevertheless,these could be inhibited by IGF-1 receptor monoclonal antibody in a dose-dependent manner.ConclusionElevated expression of SCF may be induced by IGF-1 through proliferation of SMC.
