Study on CIX-M type ESBLs-producing Escherichia coli and Klebsiella pneumoiae in Guangzhou
10.3760/cma.j.issn.1009-9158.2009.10.007
- VernacularTitle:广州地区产CTX-M型超广谱β内酰胺酶大肠埃希菌和肺炎克雷伯菌的研究
- Author:
Chao ZHUO
;
Danhong SU
;
Hongyu LI
;
Luxia WANG
;
Kang LIAO
;
Mei WANG
;
Zhiquan ZHI
;
Zhonghui GUO
;
Yanchao WEI
;
Suina GENG
;
Guangyao JIN
;
Nanshan ZHONG
- Publication Type:Journal Article
- Keywords:
Escherichia coli;
Klebsiella pneumoniae;
beta-Lactamases;
Escherichia coli proteins;
Phenotype;
Micrebiol sensitivity tests;
Polymerase chain reaction
- From:
Chinese Journal of Laboratory Medicine
2009;32(10):1114-1119
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study phylogenies, epidemiology and genetic environment of CTX-M type of ESBLs produced by Escherichia coli and Klebsiella pneumoniae isolated from nine hospitals in Guangzhou. Methods The phylogenies of CTX-M type of ESBLs were analyzed by PCR Genetic environment of CTX-M-15 encoding gene (bla_(CTX-M-15)) were investigated by conjugation test and plasmid analysis. The clonal relationship of strains producing CTX-M-15 was determined by enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). Results A total of 361 ESBLs-producing isolates of Escherichia coli and Klebsiella pneumoniae were collected. 67.3% of ESBLs strains were detected to produce CTX-M-type ESBLs, and the commonest genotypes in Escherichia coli and Klebsiella pneumoniae were CTX-M-14 (35.4% and 28.3%), CTX-M-15(21.5% and 26.1%) EBIC-PCR products of all CTX-M-15-producing strains show 39 strains of Escherichia coli were classified into 27 genotypes while 43 strains of Klebsiella pneumoniae were divided into 30 genotypes. Furthermore, the genotypes of CTX-M-55, CTX-M-19, CTX-M-27, with ceftazidime-hydrelyzing activity, were detected in this study. The great majority of bla_(CTX-M-15) genes were found to locate on a 65 000 bp-conjugative plasmid, and there was no blaTEM-1, bla_(OXA-1), blaDSA-1 or aac (6')-Ib-cr gene coexisted on the plasmid, ISEcp1-like insertion sequences, relative to mobilization of bla_(CTX-M-15) gene, were detected in all bla_(CTX-M-15) positive strains, and the distances between the end of ISEcp1-like insertion sequences and the start cedon of bla_(CTX-M-15) were equal, with 48 base pairs. Conclusion CTX-M-14 is still the most common genotype of ESBLs in Guangzhou, but high prevalence of CTX-M-15 ESBLs hydrolyzing ceftazidime already appears in south China.
