Mechanism of the proliferation and apoptosis of keloid fibroblasts induced by 5-fluouracil:a preliminary study
10.3760/cma.j.issn.1671-0290.2009.05.010
- VernacularTitle:5-氟尿嘧啶对瘢痕疙瘩成纤维细胞增殖与凋亡机制影响的初步探讨
- Author:
Zhubin PAN
;
Xiaojing LI
;
Zongbao ZUO
;
Chaohua LIU
;
Chun WANG
- Publication Type:Journal Article
- Keywords:
Keloid;
Fluorouracil;
Smad7;
Cell apoptosis
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2009;15(5):323-326
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the of 5-fluorouracil effects on the expression of Smad7,TGF-β receptorⅠ,Bcl-2 and Bax in keloid fibroblasts.Methods After primary culture of keloid fibroblasts,4-6 passages of cells were inoculated in 5 different concentrations of 5-fluorouracil(10,20,40,80,160μmol/L)for 24,48 and 72 hours.Proliferative ability of keloid fibroblasts was detected by MTT assay.Expression of Smad 7,TGF-βreceptorⅠ,Bcl-2 and Bax in keloid fibroblasts was measured by Western blot.Results During MTT,5-fluorouracil did not affect cell viability at 24 hour at the concentration of 10 and 20 μmol/L.Compared with the control group,no significant difference was detected(P＞0.05).At other concentrations,fibroblast death was visible in each group(P＜0.01).Western blot analysis showed that the expression of Smad7 significantly decreased and the expression of TGF-β receptor Ⅰ significantly increased in the TGF-β1 group compared with the blank control group(P＜0.0 1).5-fluorouracil could significantly enhance the expression of Smad7(P＜0.01).There was a remarkable decrease of the Bcl-2 expression and marked increase of the Bax expression in different concentrations of 5-fluorouracil compared with the control group(P＜0.05).But,5-fluorouracil did not show any effect on the synthesis of TGF-β receptor Ⅰ.Conclusion 5-fluorouracil could inhibit proliferation and induce apoptosis on human keloid fibroblasts in vitro.
