Relative of Th cytokines in MRL/Ipr mice——a model for idiopathic dacryoadenitis
10.3760/cma.j.issn.0254-5101.2009.09.003
- VernacularTitle:T辅助细胞因子在特发性泪腺炎小鼠MRL/lpr中的表达研究
- Author:
Jie GUO
;
Jiang QIAN
;
Rui ZHANG
;
Yifei YUAN
- Publication Type:Journal Article
- Keywords:
Idiopathic dacryoadenitis;
MRL/lpr;
IL-4;
IL-17;
IFN-γ
- From:
Chinese Journal of Microbiology and Immunology
2009;29(9):782-785
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the distribution of IL-4, IL-17, IFN-γ in the lacrimal gland lesions and submaxillary lymph nodes of MRL/Ipr mice-a model for idiopathic dacryoadenitis, and investi-gate the possible role of T-belper cells in the disease. Methods Frozen sections of lacrimal glands and sub-maxillary lymph nodes from MRL/lpr and BALB/c mice age 3 months were stained with antibodies to the cy-tokines IL-4, IL-17 and IFN-γ, then calculated the numbers or proportions of positive cells respectively,double staining for CD4 and each cytokine was taken in lymph nodes of MRL/Ipr mice additionally. Results The major cytokine in lacrimal gland lesions is IL-4, which contrasts to little IL-17 or IFN-γ/stained(P <0.05), the numbers of cells staining for each cytokine in submaxillary lymph nodes elevate in MRL/lpr mice, and the expressions of IL-4 and IL-17 are greater than IFN-γ significantly. Conclusion Th2 may play an important role in the development of local dacryoadenitis and systemic abnormality in MRL/lpr mice,the role of IL-17 maybe different in lacrimal gland lesions and lymph nodes which need more research.