Distribution and survival of human embryonic mescnchymal stem cells in kidney of newborn mice
10.3760/cma.j.issn.1001-7097.2009.09.006
- VernacularTitle:人早期胚胎间充质干细胞在新生小鼠肾脏内的存活和分布
- Author:
Li YUAN
;
Minjuan WU
;
Yi ZHANG
;
Hongyu SUN
;
Jun XIONG
;
Chunyan LIU
;
Houqi LIU
;
Changlin MEI
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cells;
Embryo;
Kidney tubules;
Epithelial cells
- From:
Chinese Journal of Nephrology
2009;25(9):683-687
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the biological characteristics of human embryonic MSC (hMSCs) and their potential ability of differentiation, and to investigate the survival and distribution of hMSCs after transplantation into the kidney of newborn mice. Methods hMSCs were derived from 4-7 week-old embryos, then primary culture was done. The biological characteristics of hMSCs were detected by immunohistochemical methods and flow cytometry. Their differentiation potential was determined by coculture with conditioning medium. The survival and distribution of PKH-26-stained hMSCs in mice were observed by laser scanning confocal microscope. Results Flow cytometry and immunochemistry staining revealed that the expression of CD29, CD44, CD90, SH-2, OCT-4 was positive significantly, and CD34, CD45 was negative. The cells could be induced to differentiate to osteocytes and adipocytes under special conditions. After transplantation for 1 month, PKH-26-stained hMSCs still existed in the kidney of mice and co-localized in tubular epithelium by confocal microscope. Conclusion hMSCs derived from the early human embryo have the ability of proliferation and differentiation with low immunity, and may be involved in the development of renal tubule in newbem mice.
