Detection of genomic abnormalities in chronic lymphocytic leukemia by fluorescence in situ hybridization
10.3760/cma.j.issn.1009-9158.2009.10.012
- VernacularTitle:应用荧光原位杂交技术检测慢性淋巴细胞白血病的基因组异常
- Author:
Jieying HU
;
Jindong GUO
;
Ruihua FAN
;
Xudong WEI
;
Yuewen FU
;
Yongping SONG
- Publication Type:Journal Article
- Keywords:
Leukemia;
lymphocytic;
chronic;
B-cell;
In situ hybridization;
fluorescence;
Chromosome aberrations
- From:
Chinese Journal of Laboratory Medicine
2009;32(10):1138-1141
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the value of panel fluorescence in situ hybridization (panel FISH)for detection of genomic aberrations in chronic lymphocytic leukemia(CLL). Methods Five types of fluorescein-labelled DNA probes including five sequence specific probes D13S25 for 13q14. 3, RB1, p53, ATM (11 q23)and centromeric probe for chromosome (CSP12) were used to perform fluorescence in situ hybridization assays in 17 patients with CLL. Its results were compared with that obtain by conventional cytogenetic (CC)examination. Results In 17 patients with CLL, CC examination showed that only one case (1/17) was found to have chromosomal abnormality that was simultaneous trisomies 3,8 and 18, whereas panel FISH assay showed that 10 cases (10/17) were found to have genomic aberrations including deletion of D13S25 in 4 cases,deletion of ATM in 2 cases,deletion of p53 in 1 case,deletion of D13S25 combined RB1 in 1 case and 1 case with a variety of abnormalities. Conclusions Panel FISH is a useful method for detection of genomic aberration in CLL If it is combined with CC,it can obviously enhance the detection rate of chromosomal abnormalities in CLL.
