Study on the relationship between the expression of TLR7/8 in monocytes and the disease progression of HIV-1 infection
10.3760/cma.j.issn.0254-5101.2009.11.009
- VernacularTitle:单核细胞TLR7/8表达与HIV-1感染疾病进程关系的研究
- Author:
Hua NIAN
;
Wenqing GENG
;
Hualu CUI
;
Zining ZHANG
;
Min ZHANG
;
Xiaoxu HAN
;
Bin ZHAO
;
Qinghai HU
;
Hong SHANG
- Publication Type:Journal Article
- Keywords:
HIV-1;
Monocyte;
TLR7;
TLR8
- From:
Chinese Journal of Microbiology and Immunology
2009;29(11):992-996
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the expression of TLR7/8 in monocytes purified from HIV-1 infected individuals and to study its association with disease progression. Methods Sixty-three HIV-1 infected individuals and 18 normal controls were enrolled. Monocytes were purified by MACS system and RNA of them was extracted by RNA mini kit of QIAGEN company. TLR7/8 expression was tested by real-time RT-PCR with ABI7500. Results It was found that the expression of TLR7 was strongly correlated with absolute CD4 count (r =0.614, P＜0.01) , so was TLR8 (r =0.419, P＜0.01). The expression of TLR7 in slow progressor (SP) group was higher than that in HIV-1 infected patients group, AIDS patients group and normal group (P ＜ 0. 05 ) . HIV group and normal group were strongly higher than AIDS group (P ＜ 0. 05). It was no significant differentiation of expression of TLR7 between HIV infection group and normal control group. The expression of TLR8 in SP group and normal group were significantly higher than that in AIDS group (P ＜ 0. 05). The expression of TLR8 was no singnificantly difference between SP group and HIV group or normal control group, so was it between HIV group or normal control group and AIDS group. Conclusion The expression of TLR7/8 in monocytes from HIV-1 infected patients significantly correlated with disease progression.
