The distribution of nerve growth factor in rabbits' eyes after intraocular injection in two different means
10.3760/cma.j.issn.1015-1005.2009.06.17
- VernacularTitle:两种不同注射方法眼局部注射鼠神经生长因子后兔眼内药物分布
- Author:
Danning LIU
;
Zhengqin YIN
- Publication Type:Journal Article
- Keywords:
Nerve growth factor/administration & dosage;
Microinjections/methods;
Drug delivery systems/methods;
Animal experimentation
- From:
Chinese Journal of Ocular Fundus Diseases
2009;25(6):470-473
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the distribution and concentration of ~(125)I-nerve growth factor (NG-F) in rabbits' eyes after intravitreal injection and posterior juxtascleral injection.Methods Intravitreal injection(group A) and posterior juxtascleral injection (group B) were performed with the dosage of 30μg/100μl ~(125)I-NGF on left and right eyes in 45 white rabbits respectively.The γ-counts and the concentration of ~(125)I-NGF (%ID/g) of each ocular tissue was determined 15 and 30 minutes,and 1,3,6,12,24,and 48hours after injection.Results The ~(125)I-NGF diffusion in group A was faster in ocular content and ocular inner wall.The vitreous content of ~(125)I-NGF decreased gradually in group A,the curve changes in other eye tissues were normal.The concentration of ~(125)I-NGF reached the peak 3 hours after injection in aqueous humor,iris and ciliary body,retina,and choroids,but 6 hours after injection in sclera and 8 hours in cornea.The changes of concentration of ~(125)I-NGF in group B showed normal curve change.The peak time in group B were all 6 hours,in all the tissues except aqueous humor (3 hours).Except the high concentration in vitreous body caused by intravitreal injection,the concentration of ~(125)I-NGF in retina was the highest in group A.Conclusion Intravitreal injection of ~(125)I-NGF can gain higher concentration in each ocular tissue than posterior juxtaseleral injection,especially in retina.So intravitreal injection of NGF is a better ocular delivery method to treat the ocular fundus diseases.
