Effect of botulinum toxin type A injection on the expression of substance P, TGF-β1 and α-SMA in rabbit ear model of hypertrophic scar
10.3760/cma.i.issn.1671-0290.2009.06.15
- VernacularTitle:A型肉毒毒素对兔耳增生性瘢痕组织中P物质、β1转移生长因子、α平滑肌肌动蛋白的影响
- Author:
Lin WANG
;
Ningzheng TAI
;
Zhihong FAN
- Publication Type:Journal Article
- Keywords:
Botulinum toxin type A;
Hypertrophic scar;
Substance P;
Transforming growth factor beta-1;
Alpha smooth music actin A
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2009;15(6):410-413
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of botulinum toxin type A injection on the expres-sion of substance P, TGF-β1 and α-SMA in rabbit ear model of hypertrophic scar. Methods The hyper-trophic sear model was established in 24 Japanese rabbits'ears. The wounds in ventral surface of ear were divided in Group I (lateral wounds) and Group S (medial wounds), 3 wounds each side per ear, totally 72 wounds each group. The wound-healing time and the growth of scar were observed and recorded. On post-wounding day 28, the wounds were created in another 6 rabbits in the same way and the normal skin were harvested as Group C. Likewise, the scar samples in Groups I and S were harvested. The mRNA expression of substance P, TGF-β1 and α-SMA were detected quantitatively by using real-time PCR and α-SMA was also detected with Western blot. Results No difference between the ratio of healed or infec-tious wounds on post-wounding day 14. The mRNA expression of SP, TGF-β1 and α-SMA in Group I was significantly lower than Group S, but higher than those in Group C (P<0. 01 and <0. 05, respec-tively). The blot density of α-SMA and the ratio of blot density of a-SMA to β-actin in Group I were be-tween that of the other two groups. Conclusion Without delaying the wound-healing, botulinum toxin type A injection significantly decreases the expression of SP, TGF-β1 and α-SMA in rabbit ear model of hypertrophic scar.
