Rat cranial sutures in vitro: a new experimental model for studying the osteogenic effect to tensile force

VernacularTitle:大鼠颅骨体外培养矢状缝牵引成骨模型的建立
Author: Di WU ; Xingang WANG ; Ningbei YIN ; Zhenmin ZHAO
Publication Type:Journal Article
Keywords: Cranial suture; Organ culture techniques; Stress; Mechanical
From: Chinese Journal of Medical Aesthetics and Cosmetology 2009;15(6):402-404
CountryChina
Language:Chinese
Abstract: Objective To develop an in vitro model for cranial suture of neonatal SD rat. Methods The parietal hones with the sagittal suture were removed from SD rats (19-days old) for organ culture. In the experimental group, tensile force 3.92×10~(-3) N (0.4 g) was applied by helical springs, whereas no tension (0 N) was set in control group. These explants were observed under inverted microscope. At the end of the incubation period for 24 hours, general conditions were observed under inverted microscope and histological conditions were observed after hematoxylin and eosin stain. Results Under inverted micro-scope, sutures had no obvious changes in control group, whereas sutures were enlarged gradually in the experimental group. With histological observation, sutures developed normally in control group, but in experimental group, osteohlasts and capillary vessels proliferated actively in the suture. Conclusions In vitro model of cranial suture can be cultured and grown successively.