Use of IFN-γ release assay in latent tuberculosis infection diagnosis in general and HTV-infected populations
10.3760/cma.j.issn.0254-5101-2009.11.022
- VernacularTitle:结核抗原特异性IFN-γ ELISPOT检测技术用于诊断HIV感染者人群中结核潜伏感染的研究
- Author:
Hui WANG
;
Yan TAN
;
Xiuyun ZHU
;
Mingfeng LIAO
;
Jieyun ZHANG
;
Yan LIU
;
Shuiteng LIU
;
Lukun ZHANG
;
Yang ZHOU
;
Yingxia LIU
;
Boping ZHOU
;
Xinchun CHEN
;
Hongzhou LU
- Publication Type:Journal Article
- Keywords:
Human immunodeficiency virus;
Latent tuberculosis infection;
IFN-γ release assay;
Enzyme-linked immunospot assay;
Tuberculin skin test
- From:Chinese Journal of Microbiology and Immunology
2009;29(11):1037-1041
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the validity of a newly developed in-house ELISPOT IFN-γ release assay (IGRA) for the detection of latent tuberculosis infection among HIV infected individuals. Methods In-house ELISPOT assay were performed, together with a tuberculin skin test in 205 health controls and 110 HIV infected individuals , who had no signs of active tuberculosis at time of enrolment . Results Using the ELISPOT assay, positivity rates for the 205 health controls, 110 HIV infected individuals and 47 AIDS patients on highly active antiretrovial therapy (HAART) were 7. 3% , 24.5% , 29. 8% , respectively. These results indicated that the positive rates obtained from HIV infected individuals (include patient on HAART) was significantly higher than health controls( P ＜ 0.001). We found no significant correlation between the CD4 cell count and positivity of ELISPOT assay (P ＞0.05 ). The proportion of subjects with a positive response to ELISPOT assay were higher than the proportion of tuberculin skin test(TST) responders(P＜0.0001) in HIV infected individuals. Conclusion Our study indicates that IGRA using M. tuberculosis specific antigens are likely to retain their validity for the diagnosis of LTBI among HIV positive individuals.
