The effect of arsenic trioxide on metastasis and invasion of human osteosarcoma cell

VernacularTitle:三氧化二砷抑制骨肉瘤转移和侵袭的体外研究
Author: Tingting REN ; Wei GUO ; Xinchang LU ; Yi YANG
Publication Type:Journal Article
Keywords: Osteosarcoma; Arsenicals; Migration; Neoplasm invasivenes; Matrix metalloproteinase 9
From: Cancer Research and Clinic 2009;21(12):796-798
CountryChina
Language:Chinese
Abstract: Objective To study the effect and mechanism of arsenic trioxide on the motility, metastasis and invasion of osteosarcoma in vitro. Methods Wound healing assay, migration assay, invasion assay and Western-blot were performed to study the effect of arsenic trioxide on metastasis of osteosarcoma. Results Through screening MNNG cell was selected to perform the following research. After treatment of As_2O_3, the ability of MNNC cell flattening and spreading along the edges of the wound was inhibited, and the number of MNNG cells with migration and invasion in As_2O_3 - treated group was significantly less than in control group. Arsenic trioxide treatment also resulted in down-regulation of MMP-9. Conclusion This study is the first to report the effectiveness of arsenic trioxide as an inhibitor of osteosarcoma migration and invasion and the mechanism may be down-regulation of MMP-9.