Epithelial -mesenchymal transition induced by high glucose in mice podocyte cell line
10.3760/cma.j.issn.1001-7097.2009.11.012
- VernacularTitle:高糖诱导足细胞发生上皮-间叶细胞转分化
- Author:
Feng BAI
;
Weinan YU
;
Ping WEN
;
Xiaohua WANG
;
Li FANG
;
Hongdi CAO
;
Junwei YANG
;
Ruoyun TAN
- Publication Type:Journal Article
- Keywords:
Podocytes;
Diabetic nephropathies;
Kidney glomerulus;
Highglucose;
Epithelial-mesenchymal transition
- From:
Chinese Journal of Nephrology
2009;25(11):862-866
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the possible mechanism of glomerular injury in diabetes mellitus by determining whether epithelial-mesenchymal transition (EMT) is caused by high glucose in mice podocytes. Methods Using mice glomerular podocyte cell line as an in vitro system, podocytes were incubated with glucose(12.5 mmol/L, 25 mmol/L, 50 mmol/L) and mannitol (50 mmol/L) for 36 hours. Then the cells were collected and expression of alpha-smooth muscle actin(α-SMA), fibronectin (FN), CD2 associated protein (CD2AP) and Wilms' tumor 1 gene (WT-1) was detected by Western blot and indirect immunofluorescence staining. Results Under low glucose (5.6 mmol/L) and mannitol (50 mmol/L) condition, there were high expression of CD2AP and WT-1, and low expression of α-SMA and FN in mice podocytes. After 36 hours treatment with high glucose (12.5 mmol/L), the expression of α-SMA and FN in podocytes was significantly increased, and the expression of α-SMA and FN was further up-regulated with the increase of glucose dosage (25, 50 mmol/L). The indirect immunofluorescence staining revealed the similar result, and the percentage of positive α-SMA cells was also increased compared with low glucose and mannital group (P<0.05). Meanwhile, Western blot showed that high glucose could down-regulate the expressions of CD2AP and WT-1 in a dose-dependent manner. Conclusion EMT may be a potential pathway leading to podocyte dysfunction and glomerular injury under high glucose conditions.
