The effects of HSP70/CD80 DNA vaccine on airway inflammation and hyperresponsiveness of asthmatic mice
10.3760/cma.j.issn.0254-5101.2009.11.005
- VernacularTitle:HSP70/CD80嵌合DNA疫苗对哮喘小鼠气道炎症和气道高反应性的作用
- Author:
Zhengyan CHENG
;
Xiaoling SHI
;
Yan LI
;
Guoping LI
;
Shining LI
;
Sen ZHONG
;
Zhuang CHEN
- Publication Type:Journal Article
- Keywords:
HSP70;
CD80;
Asthma;
Airway inflammation;
Airway hyperresponsiveness
- From:Chinese Journal of Microbiology and Immunology
2009;29(11):981-986
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of HSP70/CD80 DNA vaccine on the airway inflammation and hyperresponsiveness of asthmatic mice. Methods Forty female healthy BALB/c mice were randomly divided into 4 groups; saline group, asthma group, pcDNA3. 1 plasmid control group, and prevention group with HSP70/CD80 DNA vaccine, with 10 mice in each group. The mice were immunized by intramuscular( i. m. ) injection with HSP70/CD80 DNA vaccine before sensitization and challenge with ovalbumin. Then, the murine model of allergic asthma was made with injection of ovalbumin intraperitoneal ( i. p. ) , and inhalation of ovalbumin. Before mice were sanctified, their airway hyperresponsiveness( AHR) was measured. After mice were sanctified, bronchoalveolar lavage fluid( BALF) was obtained and cytokine IL-13 and IFN-γ were measured. And the lung histology and histochemistry were examined. Results Compared with mice in asthma and pcDNA3. 1 group, mice in vaccine group showed significantly reduced airway inflammation (P＜0. 05) and AHR (P＜0. 05). IFN-γ content in BALF were increased in mice from vaccine group compared with the asthma group and the pcDNA3. 1 group ( P ＜0. 05) , and IL-13 content in BALF were decreased. Conclusion HSF70/CD80 DNA vaccine can reduce airway inflammation and hyperresponsiveness in asthmatic mouse and this chimerical plasmid could be a candidate vaccine to prevent asthma.
