The prevalence and genotype of human parvovirus B19 in blood products
10.3760/cma.j.issn.0254-5101.2009.11.020
- VernacularTitle:我国血液制品中人细小病毒B19污染情况及基因型的初步研究
- Author:
Yu WU
;
Yansheng GENG
;
Jingzhou WANG
;
Yongchao ZHANG
;
Chenyan ZHAO
;
Shufang MENG
;
Defu LI
- Publication Type:Journal Article
- Keywords:
Human parvovirus B19;
Factor Ⅷ concentrate;
Plasma pools;
Genotype
- From:Chinese Journal of Microbiology and Immunology
2009;29(11):1031-1034
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the prevalence and genotype of human parvovirus B19 virus among blood products and plasma pools in China. Methods B19 DNA derived from 16 lots of factor Ⅷ concentrate produced by 4 manufactures and 10 lots of plasma pools were detected by nested PCR. Phylogenetic comparison of the partial B19 sequences obtained from positive samples were performed by direct sequencing. Results Twelve of sixteen lots of factor Ⅷ concentrate and all of ten lots of plasma pools were contaminated by B19 DNA. By comparing the partial B19 sequences,all the isolated viruses were genotype Ⅰ and their nucleotides were high conserved with homology of 98. 3%-100%. Conclusion B19 genotype Ⅰ DNA has been detected in high prevalence in factor Ⅷ concentrate and plasma pools. The genetic diversities were shown to be very low.
