The detection on the p24 gene fragment of Borna disease virus in brain tissues of animals in Ili, Xin-jiang
10.3760/cma.j.issn.0254-5101.2010.01.010
- VernacularTitle:新疆伊犁地区动物脑组织博尔纳病病毒p24基因片段的检测
- Author:
Feng HE
;
Yuxing FENG
;
Houchao SUN
;
Zicheng HU
;
Hongbo XU
;
Mingming XU
;
Qunling ZHAN
;
Yongbo HU
;
Ge JIN
;
Yingying ZHANG
;
Leilei LI
;
Peng XIE
- Publication Type:Journal Article
- Keywords:
Borna disease virus;
Fluorescence quantitative nest RT-PCR;
Brain tissue;
Ili,Xin-jiang area
- From:
Chinese Journal of Microbiology and Immunology
2010;30(1):31-35
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the epidemiology characterization of Borna disease virus (BDV) in animal brain in Ili, Xinjiang, and to find out the potential infection of the Borna disease virus to prevent its outbreak. Methods The BDV p24 gene of animal brain tissues in Ili including 200 horses, 75 donkeys and 100 shepherd dogs was detected by fluorescence quantitative nest reverse transcriptase polymer-ase chain reaction(FQ-nRT-PCR). GFP-p24,pMD-19 plasmid contamination was excluded from positive products. Clone sequencing was used to analyze the homology of gene and amino acid sequence. Results BDV p24 gent was found in 3 Ili horses, 4 Ili donkeys and 9 shepherd dogs, and the positive ratio is 1.5%, 5.3% and 9.0%, respectively. The GFP-p24,pMD-19 were not found in BDV p40 gene and plasmid stand-ard. The sequence of BDV p24 amplification production was totally the same as He/80 virus strain. Conclu-sion Natural infection of BDV may exist in the animals(horses, donkeys and dogs)in Ili, and the epidem-ic strain of BDV in this area was homological as He/80 virus strain.
