Inhibition effect of celecoxib on the experimental choroidal neovascularization
10.3760/cma.j.issn.1005-1015.2010.01.09
- VernacularTitle:塞来昔布对实验性脉络膜新生血管的抑制作用
- Author:
Yuhua HAO
;
Jin LI
;
Xin ZHAO
- Publication Type:Journal Article
- Keywords:
Choroidal neovascularization/drug therapy;
Cyclooxygenase 2 inhibitors/therapeutic use;
Cyclooxygenase 2 inhibitors/administration & dosage;
Disease models,animal
- From:
Chinese Journal of Ocular Fundus Diseases
2010;26(1):32-36
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the inhibition effect of selective cyclooxygenase-2 inhibitor (celecoxib) on the experimental choroidal neovascularization (CNV).Methods Thirty 8-10 weeks old healthy male Brown-Norway(BN) rats were randomly divided into the control,laser and celecoxib group,with 10 rats in each group.At the dosage of 50 mg/kg,celecoxib was garaged twice per day.After 7 days,experimental CNV was induced by Krypon laser on laser group and celecoxib group.Fundus fluorescein angiography (FFA) was performed on days 3,7,14,21,30 after laser photocoagulation.On days 21 after photocoagulation,5 rats in each group were sacrificed and the relative thickness of CNV membranes,the expression of COX-2,vascular endothelial growth factor(VEGF) and matrix metalloproteinase-2 (MMP-2) Were studied by histopathologic or immunohistochemistry examination.Results On days 21 after photocoagulation,the incidence of CNV in the celecoxib group is significantly lower than that in the laser group (X~2=7.1068,P=0v0077);the relative thickness of the CNV membranes in the celecoxib group is reduced 41.38% compared to the laser group,the difference is statistically significant (t=16.7600,P=0.0000).COX-2,VEGF and MMP-2 expression in the CNV membrane of celecoxib group were significantly lower than in control group (t=5.7100,5.8400,8.0200;P=0.0000);the COX-2,VEGF and MMP-2 expressions in choroid and retina of control group were weak.Conclusion Prophylactic celecoxib can reduce the expression of VEGF and MMP-2 by inhibiting COX-2,and prevent the CNV induced by laser photocoagulation.
