Changes of reactive oxygen species levels in the AGS cells induced by Helicobacter pylori extracts

VernacularTitle:幽门螺杆菌提取液诱导AGS细胞内活性氧类物质水平的变化
Author: Xuewen HUANG ; Ruihua LUO ; Qi ZHAO ; Lili HUANG ; Xianyuan AN ; Lanjing ZHAO ; Jie WANG ; Yuzheng HUANG
Publication Type:Journal Article
Keywords: Helicobacter pylori; VacA protein; CagA protein; Reactive oxygen species
From: Chinese Journal of Digestion 2010;30(1):37-41
CountryChina
Language:Chinese
Abstract: Objective To investigate the relationship between Helicobacter pylori (H. pylori) infection and concentration of reactive oxygen species (ROS) in AGS cells. Methods AGS cells were cultured with either Hp11638 (CagA~+ , VacA~+ ) extract or Hp11638 mutant (CagA~+ , VacA~-) extract for 48 hours, then the cells and supernatants were collected. The concentration of ROS in AGS cells was measured by flow cytometry. The eytochrome C reduction was detected by spectrophotometer at 550 nm. Results The ROS levels in the AGS cells were correlated with two H. pylori strains in a concentration- and time-dependent manner. The ROS levels in AGS cells treated with Hp11638 extract in different concentrations or times were correspondingly higher than those treated with Hp11638 mutant extract. Similar results were found in examination of cytochrome C reduction. Conclusion The elevation of ROS in AGS cells is related to effects of H. pylori proteins, and the VaeA protein involves in the process.