Activation of CD40-CD154 co-stimulatory pathway and its correlation with disease activity in patients with inflammatory bowel disease
10.3760/cma.j.issn.1007-5232.2010.02.009
- VernacularTitle:炎症性肠病患者CD40-CD154共刺激途径的激活及其与内镜下疾病活动程度的相关性研究
- Author:
Jun SHEN
;
Zhihua RAN
;
Qing ZHENG
;
Jinlu TONG
;
Qing CAI
;
Mingming ZHU
;
Meilan HUANG
;
Tianrong WANG
- Publication Type:Journal Article
- Keywords:
Colitis,ulcerative;
Complement pathway;
Crohn's disease
- From:
Chinese Journal of Digestive Endoscopy
2010;27(2):80-84
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of CD40-CD154 co-stimulatory pathway in peripheral circulation and intestinal mucosa in patients with inflammatory bowel disease (IBD),the difference between the expression of CD40-CD154 in patients with IBD and that in healthy controls,and the correlation between CD40-CD154 levels and disease activity. Methods A total of 62 patients with Crohn's disease (CD),64 patients with ulcerative colitis (UC) and 56 healthy controls were enrolled. Enzyme-Linked Immuno Sorbent Assay (ELISA),SYBR-green real time PCR and immunohistochemical assay were respectively applied to evaluate expression of CD40-CD154 in plasma,mononuclear cells of peripheral blood and intestinal mucosa. Results Levels of CD40 (P=0. 000) and CD154 (P=0. 001) in plasma,mononuclear cells of peripheral blood and intestinal mucosa were significantly higher in patients with CD and UC than in healthy controls. However,no correlation between disease activity and CD40-CD154 expression in peripheral circulation or intestinal mucosa was detected (P ＞ 0. 05 ). Conclusion CD40-CD154 pathway activation is found in plasma,peripheral blood mononuclear cells and intestinal mucosa in patients with IBD,but is not correlated with disease activity.
