Establishment of plaque reduction assay and its application in the susceptibility testing against influenza virus in vitro
10.3760/cma.j.issn.1009-9158.2010.01.005
- VernacularTitle:蚀斑减数试验的建立及在体外抗流感病毒药物敏感性试验中的应用
- Author:
Ying ZHANG
;
Anping NI
;
Jingtao CUI
;
Qian LI
;
Yaling DOU
;
Yeli WU
;
Jianwei WANG
- Publication Type:Journal Article
- Keywords:
Orthomyxoviridae;
Plaque assay;
Microbial sensitivity tests;
Oseltamivir;
Amantadine;
Ribavirin;
Badix isatidis
- From:
Chinese Journal of Laboratory Medicine
2010;33(1):20-24
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish plaque reduction assay and evaluate the activities of oseltamivir (tamiflu),amantadine,ribavirin and herb radix isatidis against influenza virus in vitro.Methods Plaque reduction assay was used to determine IC_(50) values of four studied drugs above in this susceptibility testing in which 8 clinical isolates(three influenza A virus isolates and five influenza B virus isolateds)were inoculated and tested.Results By testing of 8 clinical isolates of influenza virus A and B isolated between the year 2001 to 2008,oseltamivir and amantadine were found to be sensitive to influenza A virus with IC_(50) of 0.064 -0.128 mg/L and 0.5 mg/L,respectively.However,ribavirin(IC_(50)>8 mg/L)was not found to be sensitive,and herb radix isatidis had totally no activities.Unfortunately.all four studied drugs were not found to have activities against influenza B virus in vitro.Conclusions It Was indicated that oseltamivir and amantadine.but not ribavirin and herb radix isatidis.are sensitive to influenza A virus.All four studied drugs were not found to have activities against influenza B virus in vitro.
