Features of human umbilical cord mesenchymal stem cells cultured on silk fibroin porous scaffolds in vitro

VernacularTitle:人脐带间充质干细胞与蚕丝素多孔支架的体外复合培养
Author: Yi LIU ; Hongtao XIAO
Publication Type:Journal Article
Keywords: Umbilical cord; Mesenchymal stem cells; Silk fibroin; Scaffolds t Tissue engineering
From: Chinese Journal of Medical Aesthetics and Cosmetology 2010;16(1):45-48
CountryChina
Language:Chinese
Abstract: Objective To observe the attachment,shape,function and activity of human umbilical cord mesenchymal stem cells cultured on silk fibroin porous scaffolds in vitro,and to provide experimental foundation for the choice of scaffold in the study of adipocyte tissue engineering.Methods These silk fibroin porous scaffolds were subsequently seeded with hUCMSCs and cultured in vitro.The growth and function of hUCMSCs were observed and measured with fluorescence inverted microscopy,scanning electronic microscopy and MTT methed. Results hUCMSCs were fixed on silk fibroin porous scaffolds 1 or 2 days later,and multiplicative growth was observed on the 5th to 7th day.After about 10 days,the microholes of the scaffolds were overlayed by hUCMSCs.Scanning electronic microscopy and fluorescence inverted microscopy showed that cells adhered to scafold well and there was a lot of extra cellular matrix surrounding cells. Conclusion Silk fibroin porous scaffolds are ideal for attachment,growth,function maintenance and activity of hUCMSCs.and the scaffolds can be used as natural scaffolds for hUCMSCs in 3D culture.