Enhancement of the immune response of a novel DNA vaccine encoding conserved NS3 and Core fusion gene of HCV injected by intradermal electrotransfer in mice

VernacularTitle:表达丙型肝炎病毒NS3和Core融合蛋白基因的DNA疫苗免疫方案优化
Author: Xiao YIN ; Jian LU ; Wenjie TAN ; Yao DENG ; Jie GUAN ; Ruiguang TIAN ; Wen WANG ; Hong CHEN ; Shengli BI ; Li RUAN
Publication Type:Journal Article
Keywords: Hepatitis C virus (HCV); DNA vaccine; Intradermal electrotransfer
From: Chinese Journal of Microbiology and Immunology 2010;30(1):41-45
CountryChina
Language:Chinese
Abstract: Objective To characterize the immunogenicity in gene immunization of the conserved regions of hepatitis C virus(HCV) based on different delivery strategies. Methods We first constructed a novel DNA vaccine encoding a fusion gene(from partial NS3 and Core) of HCV. Then we compared different protocols based on naked DNA injection twice or DNA injection with gene electrotransfer(GET) in BALB/c mice. The immune response was measured by antibody ELISA and by IFN-gamma ELISPOT. Results Our data showed that a protocol based on intradermally injection of DNA with optimal GET induced the strongest humoral and cellular immunity, and DNA with GET induced a substantially higher anti-NS3/Core T cell re-spoase than naked DNA injection. Conclusion Our data suggest that DNA vaccines encoding NS3/Core fu-sion protein of HCV immunized by the present strategy could merit further study in the context of future prophylactic and therapeutic HCV T cell based vaccines.