Increased expression of Siglec-1 and cytokines by stimulation of oxidized low-density lipoprotein on RAW264.7 cells
10.3760/cma.j.issn.0254-5101.2010.01.014
- VernacularTitle:氧化型低密度脂蛋白对RAW264.7细胞Siglec-1表达及分泌细胞因子的影响
- Author:
Yisong XIONG
;
Ye ZHU
;
Huiqi LU
;
Chang LI
;
Zhijun HAN
;
Renqian ZHONG
- Publication Type:Journal Article
- Keywords:
Atherosclerosis;
Oxidized low-density lipopretein(ox-LDL);
Siglec-1;
RAW264.7
- From:
Chinese Journal of Microbiology and Immunology
2010;30(1):51-55
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of sialic acid-binding immunoglobulin-like lectin-one (Siglec-1) in the process of atherosclerotic inflammation induced by oxidized low-density hpepmtein (ox-LDL). Methods Ox-LDL was synthesized by oxidization of native LDL and different concentration of ox-LDL was added to the culture medium of RAW264.7. Forty-eight hours later, cells and supernatants were collected separately. The expression of Siglec-1 protein and mRNA were measured by flow cytometry(FCM) and real-time quantitative RT-PCR, respectively. The levels of monocyte chemotactic protein-1 (MCP-1), macrophage inflammatory protein-1α(MIP-1α) and IL-8 in supernatants were determined by ELISA. Re-sults By the stimulation of ox-LDL, Siglec-1 protein and mRNA on RAW264.7 cells were significantly in-creased, meanwhile, the cytokines levels in culture supematants were significantly higher than that in the control group. And both Siglec-1 expression and cytokine secretion were ox-LDL dose-dependent. Conclu-sion Ox-LDL can increase Siglec-1 protein and mRNA expression and some inflammatory cytokines secre-tion on RAW264.7 cells in a dose-dependent manner. Manifested by enhanced Siglec-1 expression, the acti-vated macmphages may take a part in the development and progression of atherosclerosis.
