Aberrant promoter methylation of SFRP1 and SFRP2 gene in gastric cardia adenocarcinoma
10.3760/cma.j.issn.1006-9801.2009.12.003
- VernacularTitle:Wnt拮抗基因SFRP1、SFRP2启动子区甲基化与贲门腺癌关系的研究
- Author:
Zhiming DONG
;
Fuli WANG
;
Guoliang JIN
;
Wei GUO
;
Yanli GUO
;
Shijie WANG
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Genes;
Methylation;
SFRP1;
SFRP2
- From:
Cancer Research and Clinic
2009;21(12):799-802
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the promoter methylation status of SFRP1 and SFRP2 gene in gastric cardia adenocarcinoma (GCA). Methods Methylation specific PCR (MSP) method was used to examine the methylation status of the 5' CpG island of SFRP1 and SFRP2 gene in tumors and corresponding normal tissues. Results Methylation frequencies of SFRP1 and SFRP2 gene in tumor specimens were 87.2 % (82/94) and 83 %(78/94), which was significantly higher than that in corresponding normal tissues (14.9 % and 55.3 %, respectively) (P <0.001). Methylation frequencies of SFRP1 in lymph node metastasis group (96.4 %) was significantly higher than that in no lymph node metastasis group (73.7 %). Methylation frequencies of SFRP1 and SFRP2 gene in poor differentiation group were all higher than that in moderate and poor-moderate differentiation groups, but both of them did not show significant difference(P >0.05). 63 cases of GCA showed both of SFRP1 and SFRP2 gene simultaneous methylation, which including 36 cases of lymph node metastasis group, 27 cases of no lymph node metastasis group. Simultaneous methylation frequencies of SFRP1 and SFRP2 gene in lymph node metastasis group was higher than that in no lymph node metastasis group, poor differentiation group was higher than that in moderate and poor-moderate differentiation groups, but both of them did not show significant difference (P >0.05). Conclusion Promoter methylation of SFRP1 and SFRP2 might be related with oncogenesis of GCA and hypermethylation of SFRP1 gene might be related with the malignant behavior of GCA.
