Regulation of B-lymphocyte stimulator expression levels in response to IFN-γ and IL-6

VernacularTitle:IFN-γ和IL-6对多发性骨髓瘤B淋巴细胞刺激因子表达变化的调控研究
Author: Jiang PU ; Yueguo WANG ; Hongbing NI ; Mei WANG ; Hongxiang YUAN ; Shaoqing JU
Publication Type:Journal Article
Keywords: Multiple myeloma; B-lymphocyte stimulator; Nuclear faetor-kB; Mitogen activatedprotein kinase
From: Chinese Journal of Microbiology and Immunology 2009;29(4):351-355
CountryChina
Language:Chinese
Abstract: Objective To investigate the regulation of B-lymphocyte stimulator(BLyS) levels in response to IFN-γand IL-6. Methods Flow cytometry, quantitative polymerase chain reaction, ELISA and Western blot were applied to examine the expression level of BLyS in response to IFN-γ and IL-6 . Results IFN-γand IL-6 induced BLyS expression in KM3 cells. After treated with BAY11-7082, an IkB-α phospho- rylation inhibitor, the up regulation of BI,yS induced by IFN-γ was completely inhibited. Inhibiting the nu-clear faetor-kB (NF-kB) and mitogen activated protein kinase(MAPK) activation in KM3 cells reduced BLyS protein and gene expression. Conclusion MAPK and NF-kB pathways are involved in the regulation of BLyS expression, which suggests that MAPK and NF-kB might be used for the treatment of multiple mye- loma.