Effects of vitamin E on the expression of hyaluronic acid synthetase-2 gene in human dermal fibroblasts in vitro
10.3760/cma.j.issn.1671-0290.2009.04.001
- VernacularTitle:维生素E对体外培养人皮肤成纤维细胞透明质酸合成酶-2基因表达的影响
- Author:
Liyun FAN
;
Quanzhong LIU
- Publication Type:Journal Article
- Keywords:
Vitamin E;
Hyaluronic acid synthetase-2 (HAS-2);
Fibrolasts;
Skin aging
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2009;15(4):217-219
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of vitamin E on delaying skin aging by observ-ing the expression of hyaluronic acid synthetase-2 (HAS-2) in human dermal fibroblasts in vitro. Methods Human skin fibroblasts were cultured in vitro, and these fibroblast cells were then divided into 3 groups: different concentration of vitamin E (0, 0.1 × 10-10, 1 ×109mol/L) was added in the medium in the different group. 24 hours later, the fibroblasts were collected, RNAs extracted, and then amplified by RT-PCR. The PCR product was determined by agarose gel electrophoresis, to analyze the level of HAS-2 mRNA expression. Results RT-PCR showed the lever of HAS-2 mRNA was higher in the low-dose group than the control group, with significant difference (P<0.05) ; the lever of HAS-2 mRNA was higher in the high-dose group than control group, with significant difference (P<0.05). There was no significant difference in the lever of HAS-2 mRNA between the low-dose group and the high-dose group. Conclusions Vitamin E can enhance the expression of hyaluronic acid synthetase-2 mRNA, may increase the synthesis of HAS in skin fibroblasts and increase water content in the skin, so that it might reverse or delay the skin aging.
