Membrane protein proteomic analysis of in vivo induced carbapenemases resistance Acinetobacter baumannii
10.3760/cma.j.issn.0254-5101.2009.09.006
- VernacularTitle:体内诱导耐碳青霉烯类鲍曼不动杆菌膜蛋白质组学研究
- Author:
Liulin LUO
;
Chunmei YING
;
Xiaofei JIANG
;
Yangqin YE
;
Yaping WANG
;
Haomin ZHANG
- Publication Type:Journal Article
- Keywords:
Acinetobacter baumannii;
C.arbapenem;
Resistance;
Membrane protein;
Proteomic
- From:
Chinese Journal of Microbiology and Immunology
2009;29(9):796-800
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of outer membrane protein in clinical isolated car-bapenem resistance Acinetobacter baumannii. Methods Carbapenem resistance and sensitive strains were collected from the same patient. After MIST and REP-PCR analysis, carbapenemases were detected by isoe-lectric focusing. Different expressed membrane proteins were identified by two-dimension electrophoresis and mass spectrometry analysis. We also used efflux pump inhibitor PAβN(Phe-Arg-β-naphthylamide) to con-firm the phenotype. Results Carbapenem resistance and sensitive strains were attributed to the same pat-tern. At positions of P17.6 and P19.0, two β-lactamases were expressed in two investigated strains, no cabapenemases were detected. Six differential expressed membrane proteins were identified, a 34 × 10~3 membrane protein that was confirmed by efflux pump inhibitor PAβN experiment (imiponem MIC decreased from far above 32 μg/ml to 8μ/ml) and OprD and CarO. Conclusion Up-regulation of exported protein accompanied with down-regulation of OprD and CarO other than carbaponemases are responsible for carbap-enem resistance in A. baumannii.
