Significance of CCL20 expression in liver biopsies of chronic hepatitis B patients
- VernacularTitle:趋化因子CCL20在慢性乙型肝炎活检组织中的表达及意义
- Author:
Xianan SHAO
;
Sidong XIONG
;
Wei XU
;
Fuhua YUAN
;
Ruibin LI
;
Yong WANG
;
Zhihe CHEN
;
Changjiang XU
- Publication Type:Journal Article
- Keywords:
Hepatitis B,chronic;
Chemokine CCL20;
Biopsy;
Reverse transcriptase poly merase chain reaction
- From:
Chinese Journal of Laboratory Medicine
2008;31(8):864-867
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the correlation of histologicalactivity(HAI) of chronic hepatitis B (CHB) with CCL20 expression, and to investigate the impact of CCL20 expression in CHB infection. Methods On the basis of established competitive quantitative RT-PCR with an internal standard, the expression of the CCL20 in the hepatocytes in different infected patterns of HBV infected cells and liver biopsies were quantified and at the same time its correlation to HAI were explored. Results In the cell levels, the expression quantity of CCL20 in control cells (HepG2), persistent HBV infected hepatocytes( HepG2. 2. 15) are (2. 65 ± 0. 02) pg/106 cells, ( 1.22± 0. 04) pg/106 cells, respectively. There were significantly differences between them ( t = 39. 66, P < 0. 01 ). The expression of CCL20 was enhanced in hepatocytes stimulated by PMA but their expression pauern was not changed. Moreover, CCL20 expression in liver biopsies with CHB was (3.54 ± 0. 65 ) pg/20 mg and CCL20 expression in control groups was ( 8. 74±0. 56) pg/20 mg. The expression of CCL20 between two groups was different (t =30. 09,P <0. 01 ) and correlation lied in between HAI and CCL20 expression in liver biopsies of CHB patients ( r = 0. 675, P =0. 023 ). Conclusion CCL20 expression was down-regulated and it was correlated to HAI of liver biopsies in CHB patients.
