High-glucose up-regulates the expression of fibronectin mediated by integrin-linked kinase in renal tubular epithelial cells
- VernacularTitle:整合素连接激酶介导的高糖诱导肾小管上皮细胞纤连蛋白的表达
- Author:
Mingxia XIONG
;
Ruoyun TAN
;
Feifei ZHANG
;
Xiaohua WANG
;
Li FANG
;
Wei FAN
;
Xiaoyun WANG
;
Junwei YANG
- Publication Type:Journal Article
- Keywords:
High-glucose;
Kidney tubule;
Integrin-linked kinase;
Fibronectin
- From:
Chinese Journal of Nephrology
2008;24(8):571-574
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between high-glucose-induced fibronectin(FN) expression and up-regulation of integrin-linked kinase(ILK) in human kidney tubular epithelial cells (HKC) and kidney of CD-1 mice. Methods Cultured human kidney tubular epithelial cells and streptozotocin (STZ)-indueed diabetic model of CD-1 mice were enrolled in this study.Western blot was used to detect the expression of FN and ILK.The kinase dead ILK plasmid (pCMV-kdlLK) were transferred to HKC. Results Four weeks after injection of STZ,CD-1 mice had higher blood glucose level as compared to the control [(20.3±2.7) mmol/L vs (6.1±1.4) mmol/L,P＜0.01].Meanwhile,expression of FN and ILK was significantly increased in diabetic mice as compared to the control (P＜0.01).There was positive correlation between the expression of FN and ILK (r=0.899,P＜0.01).High-glucose could up-regulate FN and ILK expression in cultured HKC in a time- and dose-dependent manner.Blockage of ILK activation by pCMV-kdILK abrogated high-glucose-incuced FN expression in HKC. Conclusions Highglucose can induce FN expression through up-regulating ILK expression.Blockage of ILK activation abrogates this effect.
