The multidrug efflux pump AdeABC in clinical Acinetobacter baumannii isolates
10.3760/cma.j.issn.0254-5101.2010.02.006
- VernacularTitle:鲍曼不动杆菌临床株多重药物外排泵AdeABC的研究
- Author:
Yue WANG
;
Shiduo SONG
;
Wei QI
;
Demeng LIU
;
Yubao WANG
;
Zhe WANG
;
Wenxue GUO
- Publication Type:Journal Article
- Keywords:
Acinetobacter baumannii;
Efflux pump;
Multidrug resistance;
adeRS
- From:
Chinese Journal of Microbiology and Immunology
2010;30(2):126-129
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of active efflux pump AdeABC in clinical Acineto-bacter baumannii islates and whether this efflux pump confers resistance to antibiotics. Methods The anti-biotic susceptibility and the function of efflux pump inhibitor were tested by micro-dilution broth method. The expression of adeB was examined by RT-PCR. The controlling gene adeRS was amplified by PCR and se-quenced. Results Thirty multidrug resistance Acinetobacter baumannii isolates and 5 sensitive isolates for PCR were both abtained the expected products of adeB and adeRS. The mRNA expression of adeB in 15 multidrug resistance(MDR) isolates were positive, but there was no expression of adeB in 5 sensitive iso-lates. The mutations of adeRS existed in 2 MDR isolates. Conclusion The expression of AdeABC may in-volved in the resistant mechanisms of the clinical MDR Acinetobacter baumannii isolates.
