Purification and part of physico-chemical characterization as well as biological activity for immuno-globulin ribonucleic acid of anti-rabies
10.3760/cma.j.issn.0254-5101.2010.02.009
- VernacularTitle:抗狂犬病免疫核糖核酸的分离纯化及部分理化性质和生物学活性初探
- Author:
Lin YANG
;
Suzhi YAN
;
Jinyan ZHANG
;
Chunhong LIU
;
Ye QIU
;
Tong LI
;
Yingxin XU
;
Tianyao XIA
;
Su YAN
- Publication Type:Journal Article
- Keywords:
Anti-rabies;
Immunoglobulin ribonucleic;
Purification;
Physico-chemical character-ization;
Biological activity
- From:
Chinese Journal of Microbiology and Immunology
2010;30(2):140-143
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the preparation of specific immune RMA(iRNA) on anti-rabies and further study immunotherapy of rabies virus exposure. Methods Horses were immunized with the rabi-es virus and their livers were isolated from the horse of antiserum, from which total RNA was extracted and purified by sodium lauryisulfonate, phenol, chloroform, ethyiene glycol monomethyl ether, cetyltrimethyam-moniumbromide and alcohol. Results Pure preparation physico-chemical characterization was analyzed, and it's weight was 0.15% of weight of liver. The RNA contained 2.86% DNA and 1.16% protein. The iRNA with a maximum UV absorbance at 258 nm and A_(258/280) about 2.0. The test of RNA was positive, which had a relative molecular mas of 13.7×10~3 by high performance liquid chromatography(HPLC), and its hy-perchromic effect was 50.67%. The vesults of biological activity was showed that the rate of leucocyte adher-ence inhibition(LAI) was 41.73%, The protective rate was 50% and prolonging the life was 31.62%. Conclusion The results obtained with the practical value were identical and provide a basis on medicines of anti-rabies.
