Preliminary study of microsatellite instability and loss of heterozygosity in thymic squamous cell carcinoma
10.3760/cma.j.issn.1001-4497.2010.01.017
- VernacularTitle:胸腺鳞癌微卫星不稳定和杂合性缺失的初步探讨
- Author:
Minghai SHAO
;
Wei HU
;
Jianhua WANG
;
Hongsheng LU
;
Shilin CHEN
- Publication Type:Journal Article
- Keywords:
Thymus neoplasms;
Carcinoma squamous cell;
Polymorphism;
single nucleotide mierosateUite in-stability;
Loss of heterozygosity
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2010;26(1):45-48
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the frequency of micresatellite instability (MSI) and loss of heterozygosity (LOH) and select sensitive loci for studying microsatellite DNA imbalance in thymic squamous cell carcinoma. Methods 5 microsat-ellitc polymorphism markers and extrated DNA were selected from 9 specmiens of paired thymie squamous cell carcinoma/nor-real tissues. MSI and LOH in the specmiens of thymic carcinoma and relevant pericancerou tissues were detected by polymerase chain reaction (PCR) followed by 6% polyacrylamide gel electrophoresis(PAGE) with silver staining. Results MSI or LOH was detected in 9 thymic carcinoma tissues. The frequency of MSI or LOH was 66.7% (6/9) at loci of D6S1708, 33.3% (3/9) at TP53, 33.3% (3/9)at DM, 33.3% (3/9)at D11S988 and 0% (0/9)at D8S136, LOH at D6S1708 (5/6) was a common genetic alteration. DI1S988 had only LOH alteration. Conclusion D6S1708, TP53, DM, and D11S988 are sensi-tive loci for studying microsatellite DNA imbalance in thymic squamous cell carcinoma. Microsatellite DNA imbalance may play a certain role in occurrence and development of thymic squamous cell carcinoma, and the relationship between MSi or LOH.The linicopathological features of thymic squamous cell carcinoma needs further investigation.
