Effects of epigallocatechin gallate on growth and metastases of colonic cancer
10.3760/cma.j.issn.0254-1432.2010.01.006
- VernacularTitle:表没食子儿茶素没食子酸酯对结肠肿瘤生长与转移的作用
- Author:
Junhua YUAN
;
Hong JIANG
;
Xiaoyun YANG
;
Yanqing LI
- Publication Type:Journal Article
- Keywords:
Colonic neoplasms;
Epigalloeatechin gallate;
NF E2-related factor 2;
UDP-glucuronosyltransferase
- From:
Chinese Journal of Digestion
2010;30(1):18-23
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the preventive effects of epigallocatechin gallate (EGCG) on growth and metastases of orthotropic colonic cancer. Methods Forty BALB/C male nude mice were prepared for model of colonic cancer and then divided into control group and low-, medium- and high-dose of EGCG groups with 10 each. Except control group, the mice in other three groups were treated with 5, 10 and 20 mg·kg~(-1)·d~(-1) of EGCG. The effect of EGCG on growth and metastases of colonic cancer was observed. The histopathologic changes of liver and lung were observed with HE, and protein expression of NF-E2-related factor 2 (Nrf2) in cancerous tissue was detected by immunohistochemistry. RT-PCR was used to examine mRNA levels of Nrf2, UDP-glucuronosyhrans-ferase (UGT)1A, UGT1A8 and UGT1A10. Results In comparison with control group [(564±130) mg], the average weight of the tumor in low-, medium- and high-dose groups was (152±63) mg, (76±42) mg and (18±10)mg, respectively, with tumor inhibitory rate of 73.0%, 86.5% and 96.8%, respectively (all P value<0.05). There was a positive correlation between tumor inhibitory effect and dosage of EGCG (P<0.05). The protein expression of Nrf2 and the mRNA levels of Nrf2, UGT1A, UGTIA8 and UGTIA10 in three EGCG treated groups were increased significantly compared with control group (P<0.05), and there was a phenomenon of nuclear transcription of Nrf2. Conclusions EGCG can prevent local growth and metastases of orthotopic colonic cancer in a dose-dependent manner in nude mice. The inhibitory effect may be caused by inducing the expressions of Nrf2, UGT1A, UGT1A8 and UGT1A10 genes.
