Non-adherent bone marrow-derived mesenchymal stem cells differentiate into neuron-like cells in mice
10.3760/cma.j.issn.1007-9408.2010.02.010
- VernacularTitle:小鼠非黏附骨髓间充质干细胞分化为神经元样细胞的实验研究
- Author:
Yan WANG
;
Yanling CHEN
;
Xiaoming BEN
;
Xiaoyu ZHOU
;
Dengshun MIAO
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cells;
Cell differentiation;
Neurons;
Brain ischemia;
Mesenchymal stem cell transplantation
- From:
Chinese Journal of Perinatal Medicine
2010;13(2):123-127
- CountryChina
- Language:Chinese
-
Abstract:
Objecttve To determine whether non-adherent bone marrow-derived mesenchymal stem cells (NA-BM-MSC) can differentiate into neuron-like cells in vitro and in vivo,especially in ischemic brain of mice. Methods NA-BM-MSC of mouse were indueed with conditioned medium containing epidermal growth factor and basic-fibroblast growth factor and demonstrated by immunocytochemistry for the expression of neuron specific nuclear protein and neurofilament-200. NA-BM-MSC from β-galactosidase transgenic mice constitutively expressing β-galactosidase were transplanted into the mice model of middle cerebral artery occlusion.LacZ staining and immunobistochemistry staining were performed to detect the survival,distribution and the differentiation of the donor cells in the ischemic brain. Results NA-BM-MSC can be induced into neuron specific nuclear protein and neurofilament-200 positive cells in vitro.LacZ staining showed that NA-BM-MSC can survive in ischemic brain and express β-galactosidase.Also,numbers of the neuron specific nuclear protein positive cells in β-galactosidase-positive cells were detected in the ischemic brain with double immunohistochemistry staining. Conclusions NA-BM-BMC can be induced to differentiate into neuron-like cells in vitro,and also can differentiate into neuron-like cells in ischemic brain tissue of the mice.
