Study of the relationship between antibody level and protection induced by human papillomavirus(HPV) L2-peptide
10.3760/cma.j.issn.0254-5101.2010.02.013
- VernacularTitle:人乳头瘤病毒(HPV)L2肽免疫小鼠诱导的抗体水平与保护作用的关系研究
- Author:
Xueling WU
;
Junsheng CUI
;
Shufang MENG
;
Baowei LI
;
Chuntao ZHANG
;
Jinping FAN
;
Rongguang SHAO
;
Youchun WANG
- Publication Type:Journal Article
- Keywords:
Human papillomavirus;
L2-peptide;
Pseudovirus;
Infection model;
Neutralizing anti-bodies
- From:
Chinese Journal of Microbiology and Immunology
2010;30(2):159-163
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the protection induced by HPV-58 L2 11-200 AA in animal, and analyze the relationship between antibody or neutralizing antibody titers and the protection generated by the immunizmg agent. Methods The peptide of HPV-58 L2 11-200 AA was expressed in E. coli and the mice were immunized with the peptide after purification and adsorption with aluminum adjuvant. The protection provided by different immunizing doses was detected in the mouse model against the challenge of the pseud-ovirions of human papiilomavirus types 58. The total antibodies and neutralizing antibody titers of serum were tested with ELISA and neutralization assay against HPV-58 pseudovirus, respectively. The total antibodies or neutralizing antibody titers that can protect the mouse from infection were analyzed. Results The mice can be protected from the challenge with HPV pseudovirus when the immunizing dose was 8 μg. The neutralizing antibody can not be detected in the immune serum by neutralization assay against pseudovirus. The total anti-body level has a corresponding relationship with the protection showed in mouse model. The results of total antibodies detected by ELISA showed that when the titer of total antibodies was ≥25 000, luminescent signal can not be detected and the mice can be protected from pseudovirus infection. Conclusion HPV-58 L2 11-200 AA peptide can protect mice from pseudovirus infection. L2 peptide has a promising perspective to be a candidate vaccine and the level of total antibodies in the immune serum can be used as a surrogate for the evaluation of protection against HPV infection.
