The effect of hyperbaric oxygen on the expression of hypoxia inducible factor-1alpha mRNA in renal tissue after renal ischemia-reperfusion injury
10.3760/cma.j.issn.0254-1424.2010.03.006
- VernacularTitle:高压氧干预对大鼠肾缺血再灌注损伤肾组织低氧诱导因子-1α mRNA表达的影响
- Author:
Zhihui XIE
;
Zongping CHEN
;
Rui CAO
;
Lei YANG
;
Zuoxun CHEN
;
Guobiao LIANG
;
Li HUANG
- Publication Type:Journal Article
- Keywords:
Hyperbaric oxygen;
Ischemia-reperfusion injury;
Kidneys;
Hypoxia inducible factor-1α
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2010;32(3):182-185
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of hypoxia inducible factor-1alpha (HIF-1α) mRNA in re-nal tissue after renal ischemia-reperfusion injury (IRI), and to investigate the effect of hyperbaric oxygen (HBO) on renal IRI and its mechanism. Methods Forty-two Wistar rats were randomly divided into a normal control group(n =6), a renal IRI group (n=18) and an HBO treatment group (n=18). Renal IRI models were established in all the rats. The plasma levels of Cr in the experimental groups were then measured after 1, 3 and 5 hours. The expres-sion of HIF-1α mRNA was also detected using real-time PCR and immunohistochemistry. Kidney tissue sections were preserved for ultrastructure examination. Results (1) The average levels of plasma Cr in the renal IRI group were significantly higher than those in the control group. Compared with the renal IRI group, plasma Cr was significantly lower in the HBO treatment group. (2) The average expression of HIF-1α mRNA was significantly lower an hour after reperfusion, but significantly higher after 3 hours than in the control group. There was no significant difference by the 5th hour after reperfusion. In the HBO treatment group, HIF-1α mRNA was up-regulated significantly at the 1st and 3rd hour after reperfusion compared with the renal IRI group, but it was reduced significantly by the 5th hour after reperfusion. (3) The severity of the kidney injury increased gradually with time in the renal IRI group. After HBO treatment, however, the damage to the renal tissues decreased significantly. Conclusions HIF-1α mRNA plays an important role in the development of renal IRI. The damage to renal tissues and renal function improves significantly after reperfusion and HBO treatment through earlier priming and up-regulating of HIF-1α mRNA expression. HBO should be applied early to help prevent renal IRI.
