Inhibitory effect of Ad-hTERTp-HSV-TK/GCV system on mice ascites production planted by liver cancer cell line
10.3760/cma.j.issn.1006-9801.2010.02.010
- VernacularTitle:重组腺病毒Ad-hTERTp-HSV-TK/GCV系统对小鼠肝癌腹腔种植腹腔积液生成的影响
- Author:
Qiang YANG
;
Zhihua DENG
;
Yan LIU
;
Jing JIA
;
Suya GUO
;
Jinglong LIU
- Publication Type:Journal Article
- Keywords:
Liver neoplasms,experimental;
Gene therapy;
Kinase adenosine;
Adenoviruses.human;
Ascites
- From:
Cancer Research and Clinic
2010;22(2):101-104
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Ad-bTERTp-HSV-TK/GCV system on malignant ascites of mice and probe into its mechanism of action.Methods The SX1 inbred strain mice were injected with H22 cell line of liver cancer and were divided into 4 groups at random.The mice in each group were given corresponding treatment after 48 hours.The production of ascites and survival period were evaluated. The apoptosis rates of tumor cells were detected by FCM.Morphological changes of tumor cells were studied by electromicroscope.Results Compared with other groups.Ad-hTERTp-HSV-TK/GCV Can obviously inhibit the production of ascites(P<0.01),prolong the survival period (P<0.01),and apoptosis rate in this group (27.12±2.12)% was significantly higher than that in other groups.No obvious side effect Was found during the treatment.Conclusion Ad-hTERTp-HSV-TK/GCV system Can inhibit production of ascites and prolong the survical period of mice by inducing apoptosis of hepatoma cells,which is a safe and feasible treatment for hepatoma therapy.
