Effect of concentrations of sucrose on Streptococcus mutans gbpC expression
10.3760/cma.j.issn.0254-5101.2010.03.003
- VernacularTitle:蔗糖浓度对口腔变形链球菌gbpC基因表达的影响
- Author:
Ying JIANG
;
Tiezhu ZHANG
;
Kun CHEN
;
Jun WANG
- Publication Type:Journal Article
- Keywords:
Streptococcus mutaus;
Glucan-binding protein C (GbpC);
Adherence;
TaqMan realtime quantitative PCR
- From:
Chinese Journal of Microbiology and Immunology
2010;30(3):205-208
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the influence of concentrations of sucrose on Streptococcus tautens (S. mutans) gbpC expression. Methods Total RNA was extracted from S. mutans after cultured in 0.5%, 1.0%, 5.0% sucrose and then reversely transcripted to cDNA. By TaqMan RT-RCR technique, we checked gbpC expression. Results Compared with 0.5% sucrose, 1.0% sucrose significantly upregulated gbpC expression(P<0.05); gbpC expression of more-adherent S. mutans strains was higher than that of less-adherent strains, Conclusion The upregulation of gbpC expression by sucrose may be one of the mechanisms underlying the increased adherence; There might be some correlation between gbpC expression andS. mutans adherence.
