IL-17 as a molecular adjuvant on enhancing cellular immune responses to HIV DNA vaccination
10.3760/cma.j.issn.0254-5101.2010.03.017
- VernacularTitle:IL-17作为分子佐剂增强HIV DNA疫苗细胞免疫应答的研究
- Author:
Qiang LIU
;
Jin JIN
;
Qiang ZOU
;
Shuo ZHANG
;
Zheng DING
;
Hanqian XU
;
Zhibo MA
;
Tong ZHANG
;
Bin WANG
- Publication Type:Journal Article
- Keywords:
IL-17;
HIV;
DNA vaccine;
Molecular adjuvant
- From:
Chinese Journal of Microbiology and Immunology
2010;30(3):256-262
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate IL-17 as adjuvant effect on the humoral and cellular immune responses to HIV DNA vaccine by immunizing mice with HIV DNA vaccine plus IL-17. Methods We immunized the BALB/c mice with pGX-Env alone, or with pcDNA3-IL-17 by intramuscular injection. The immunization was performed on week 0, 2. The concentration of the anti-Env IgG, the stimulated index of T lymphocyte proliferation, and the expression of IFN-γ, IL-4 and IL-17 in CD4~+T cell and IFN-γ in CD8~+ T cell, specific in vivo cytotoxic T lymphocyte (CTL) activity were detected at week 4. Results We show here that IL-17 as a molecular adjuvant with the HIV DNA vaccine, pGX-Env, can enhance immune responses. Interestingly, IL-17 has no adjuvant effect on the responses for T cell proliferation, antibody production and expressions of IFN-γ, IL-4 and IL-17 in CD4~+ T cells, but rather on the up-regulation of IFN-γ in CD8~+ T cells and CTL in vivo significantly(P<0.05). Conclusion The data suggest that IL-17 as the molecular adjuvant may not effect the development and differentiation of CD4~+ Th cells, but directly affect on the CD8~+ T cell functions. The novel functionality of IL-17 on adaptive immunity may lead to develop effecfive HIV DNA vaccination targeted to potentiate the CD8~+ T cell functions.
