Evaluation of human telomerase RNA gem amplification in the progression of uterine cervical dysplasia to invasive cancer by FISH detection
10.3760/cma.j.issn.1009-9158.2010.03.006
- VernacularTitle:FISH检测人端粒酶RNA基因扩增预测宫颈病变发展趋势
- Author:
Lei MING
;
Ruoyu LUO
;
Pingan XIONG
;
Lingyun YANG
;
Jie ZHANG
- Publication Type:Journal Article
- Keywords:
Uterine cervical neoplasms;
Cervical intraepitheliai neoplasia;
In situ hybridization,fluorescence;
Telomerase
- From:
Chinese Journal of Laboratory Medicine
2010;33(3):219-223
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and significance of the human telomeruse gene (hTERC)in the cytologic specimens of cervix for early cervical lesion screening.Methods Cytologic samples of cervix including 120 normal persons,86 CIN patients and 14 SCC patients were analyzed for aberrations of 3q26 using a commercially available two-color FISH probe.Results Positive expression rates of hTERC gene in normal,CIN Ⅰ ,CIN Ⅱ,CIN Ⅲ and SCC samples were 1.7%(2/120),2.6%(1/38),55.6%(10/118),87.5%(14/16),100.0%(14/14),100.0%(14/14),respectively.Compared with CIN Ⅰ/CIN Ⅱ group,hTERC gene positive expressions in SCC/CIN Ⅲ group were higher significantly(P=0.01),and them were also difference between CIN Ⅰ-CIN Ⅲ group and normal controls(X~2=113.52,P ＜0.01).With progression from CIN to carcinoma,polyploidization resulting in aneuploidy and high TERC gene copy numbers in SCC group ascended significantly.Among the total abnormal cells,the number of the cells percentage which hTERC amplification signals was more than 3:3 in CIN Ⅰ ,CIN Ⅱ,CIN Ⅲ and SCC groups were 6.12%,7.98%,28.07% and 33.97%,respectively.Condusion The hTERC gene is increasingly gained with progression of CIN and may appear to be another screening testmarker for early cervical cancer screening and accessory diagnosis.
