Expression of opticin in the eyes of diabetic mice
10.3760/cma.j.issn.1005-1015.2010.02.12
- VernacularTitle:糖尿病小鼠眼opticin蛋白表达的研究
- Author:
Ying CHEN
;
Jin MA
;
Tiepei ZHU
- Publication Type:Journal Article
- Keywords:
Diabetic retinopathy/Phisiopathotogy;
Gene expression/genetics;
Diabetes mellitus,experimental
- From:
Chinese Journal of Ocular Fundus Diseases
2010;26(2):143-146
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the opticin expression in the eyes of non-obese diabetes (NOD) mice and non-diabetic NOD mice . Methods Twenty NOD mice were divided into diabetic group (experimental group) and non-diabetic group (control group). All the mice were killed by cervical dislocation method. The eyes were harvested, and the vitreous, retina and sclera were separately collected. Western blot and real-time reverse transcription-polymerase chain reaction(RT-PCR)were respectively used to determine opticin protein and OPTC-mRNA levels. Results The opticin protein level in the vitreous and retina was lower in the experimental group(t = 4.42,4.58; P = 0. 002,0. 002), but is same in the sclera between the 2 groups (t = 0. 27, P = 0. 794). OPTC-mRNA level was vitreous> retina> selera. OPTC-mRNA levels of vitreous and retina in diabetic group were significantly Iower(t = 3.30,2. 48; P= 0. 01, 0.04); there was no statistical significant on OPTC mRNA of sclera between two groups(t = 0. 27, P = 0. 80). Conclusion Expression of opticin was suppressed in retina and vitreous of diabetic mice.
