Prediction of CTL epitopes of Tat exon 1 region in HIV-1 CRF07_BC strains in China
10.3760/cma.j.issn.0254-5101.2010.02.008
- VernacularTitle:我国HIV-1 CRF07_BC流行重组毒株Tat蛋白第一外显子CTL表位预测
- Author:
Zhiming FANG
;
Hui XING
;
Zhefeng MENG
;
Kunxue HONG
;
Lingjie LIAO
;
Xiang HE
;
Jianxin Lü
;
Yiming SHAO
- Publication Type:Journal Article
- Keywords:
Human immunodeficiency virus;
Tat protein;
Variation;
Epitope
- From:
Chinese Journal of Microbiology and Immunology
2010;30(2):135-139
- CountryChina
- Language:Chinese
-
Abstract:
Objective To predict the CTL epitopes of Tat exon 1 region in HIV-1 CRF07_BC strains, which were prevailing in China. Methods Total of 236 plasma samples were from the 3rd National HIV Molecular Epidemic Survey (NMES3). All the subjects were infected with HIV-1 CRF07_BC viruses. The tat exon 1 region was amplified by reverse transcription reaction and nested polymerase chain reaction (nested-PCR), then the PCR products were sequenced. The distribution of CTL epitopes of this region were predicted by on-line software BIMAS HLA Peptide Binding Predictions and statistics software. Results To-tal of 236 CRF07_BC strains were from 16 provinces, mainly in intravenous drug asers(58.9%)and then sex(25.0%). It was showed that there were 12 CTL epitopes of 236 Tat exon 1 region of CRF07_BC strains mainly located in proline-rich region, cysteine-rich region and core-region. Those epitopes were banded by 5 HLA presenting molecules in genotype(A * 2501 ,A * 2902, B * 15,B * 5301 and Cw * 1203) and 6 HLA presenting molecules in serotype (B53, B58 ,B57 ,A3 ,A68 and Cw12). The frequency of single amino acid substitution was more than 50% in 7 CTL epitopes. Conclusion The CTL epitopes in Tat exon 1 of CRF07 _BC strains were located in different functional regions, and there were some amino acid variations in them.
