The study of basic fibroblast growth factor on ischemic vascularization flap in rat

VernacularTitle:bFGF对促进大鼠移植缺血皮瓣血管化的实验研究
Author: Yongjun HUANG ; Dong HUANG ; Yong MOU ; Weichi WU ; Huiru ZHANG ; Junwei GE ; Qinwen JIANG
Publication Type:Journal Article
Keywords: Basic fibroblast growth factor; Neovascularization; Flap; Transplantation
From: Chinese Journal of Microsurgery 2010;33(1):38-40
CountryChina
Language:Chinese
Abstract: Objective To investigate the application valve of bFGF to improve the viability of trans-plantation flap in Wistar rat. Methods Forty-eight Wistar rats were randomly divided into two groups based on the age: group A,B(1.5 month old, every group was 12 rats) and group C,D (3 month old, every group was 12 rats). After an ischemic model completed, recombinant bovine basic fibroblast growth factor (rb-bFGF) was given to groups A and C in ischemie zone by vascularization flap injection and the equality of normal saline to groups B and D. 14 days postoperatively, the muscular tissue was sent for histology, the blood vessel density was calculated by image analysis, and positive VEGF was detected by immunohistochem-istry. Results The member of capillaries and positive VEGF were more in group A than that in B, and also were morein group C than D(P < 0.05), but there were not statistic difference between group A and C (P > 0.05). Conclusion Recombinant bovine bFGF can stimulate angiogenesis and improve the ischemie vascu-larization flap of rat, which is not associated with their age.