A new approach for quantitative assessment of DNA methylation:methylation-sensitive high resolution melting
10.3760/cma.j.issn.1009-9158.2010.03.001
- VernacularTitle:DNA甲基化定量检测的新方法:甲基化敏感性高分辨率熔解分析
- Author:
Ming GUAN
- Publication Type:Journal Article
- Keywords:
DNA methylation;
Polymerase chain reaction;
Molecular diagnostic techniques
- From:
Chinese Journal of Laboratory Medicine
2010;33(3):197-200
- CountryChina
- Language:Chinese
-
Abstract:
Methylated DNA is considered as a new generation of tumor biomarker.It shows great value for evaluation of cancer risk,early detection and predicting prognosis.MS-HRM is a novel approach for quantitative assessment of methylation.It is based on the different base compositions after PCR amplification of bisulfite-modified DNA template,which give rise to different thermal properties of the PCR products originating from methylated or unmethylated target template.This article describs the advantages of this method and its role in the detection of cancer and inherit disease.The prospective application in the molecular diagnosis is also suggested.
